Resistance Monitoring and Cross-resistance to the Diamides in the Rice Stem Borer, Chilo suppressalis (Lepidoptera: Pyralidae)

doi:10.16819/j.1001-7216.2017.6124 307

Abstract

Abstract:

【Objective】 We aim to learn about the chlorantraniliprole and flubendiamide resistance development of Chilo suppressalis from the main rice-growing area in China, meanwhile, to define the difference between resistance levels to 97.3% chlorantraniliprole TC and 20% chlorantraniliprole SC and the cross-resistance between cyantraniliprole, tetrachlorantraniliprole, cyhalodiamide and chlorantraniliprole, flubendiamide in Yuyao and Xiangshan resistance populations.【Method】 The resistance levels of nineteen Chilo suppressalis populations collected from seven provinces to chlorantraniliprole and flubendiamide, the resistance levels to 97.3% chlorantraniliprole TC and 20% chlorantraniliprole SC and the cross-resistance between cyantraniliprole, tetrachlorantraniliprole, cyhalodiamide and chlorantraniliprole, flubendiamide in Yuyao and Xiangshan resistance populations, were monitored by rice seeding dipping method in 2015. 【Result】 Yuyao and Cangnan populations from Zhejiang Province have developed high level of resistance to chlorantraniliprole (141.1-fold and 135.0-fold), and Xiangshan population also showed moderate level of resistance (87.9-fold). Moreover, the three field populations showed moderate level of resistance to flubendiamide (15.0-fold to 58.7-fold). In addition, Yuyao and Xiangshan populations showed various resistance ratios to 97.3% chlorantraniliprole TC and 20% chlorantraniliprole SC in with higher resistance ratio to 20% chlorantraniliprole SC. The result showed the two populations both have reached moderate or high level of resistance to flubendiamide and chlorantraniliprole (27.6-fold to 133.6-fold), meanwhile they also have exhibited moderate or high level of resistance to cyantraniliprole, SPY-9080 and cyantraniliprole (30.3-fold to 120.7-fold).【Conclusion】 Significant variations among field populations to chlorantraniliprole and flubendiamide were observed, showing 117.6-fold and 146.8-fold differences, respectively, as well as the higher resistance ratio to 20% chlorantraniliprole SC in Yuyao and Xiangshan resistance populations and strong cross-resistance among cyantraniliprole, tetrachlorantraniliprole, cyhalodiamide and chlorantraniliprole, flubendiamide.

Key words: Chilo suppressalis, diamides, insecticide resistance, cross-resistance

摘要：

【目的】为了及时了解我国主要稻区二化螟对氯虫苯甲酰胺和氟苯虫酰胺的抗药性发展情况,同时明确对氯虫苯甲酰胺原药和制剂的抗性水平是否存在差异及二化螟对新研发的双酰胺类杀虫剂溴氰虫酰胺、四氯虫酰胺及氯氟氰虫酰胺与氯虫苯甲酰胺、氟苯虫酰胺间是否存在交互抗性。【方法】采用稻苗浸渍法测定了2015年我国7省19地二化螟田间种群对氯虫苯甲酰胺和氟苯虫酰胺的抗药性,同时测定了浙江余姚和象山两个抗性种群对97.3%氯虫苯甲酰胺原药、20%氯虫苯甲酰胺悬浮剂制剂的抗药性及其对溴氰虫酰胺、四氯虫酰胺及氯氟氰虫酰胺与氯虫苯甲酰胺和氟苯虫酰胺间的交互抗性。【结果】浙江余姚和苍南种群对氯虫苯甲酰胺已达141.1倍和135.0倍的高水平抗性,象山种群为87.9倍的中等水平抗性;这三个种群对氟苯虫酰胺均表现为中等水平抗性(15.0~58.7倍)。二化螟对97.3%氯虫苯甲酰胺原药和20%氯虫苯甲酰胺悬浮剂制剂存在抗性差异,浙江余姚和象山种群对制剂的抗性更高。交互抗性研究结果显示,对氟苯虫酰胺和氯虫苯甲酰胺均产生了中到高水平抗性(27.6~133.6倍)的象山、余姚种群,对溴氰虫酰胺、四氯虫酰胺和氯氟氰虫酰胺同样也产生了中到高水平抗性(30.3~127.0倍)。【结论】各二化螟地理种群之间对氯虫苯甲酰胺和氟苯虫酰胺抗药性差异较大,分别表现出117.6倍和146.8倍的差异,浙江余姚和象山二化螟种群对20%氯虫苯甲酰胺悬浮剂制剂抗药性更高且三种新双酰胺类杀虫剂与氯虫苯甲酰胺和氟苯虫酰胺之间存在交互抗性。

关键词: 二化螟, 双酰胺药剂, 抗药性, 交互抗性

CLC Number:

Dandan ZHAO, Liqi ZHOU, Shuai ZHANG, Rong YAO, Yunxia QIU, Congfen GAO. Resistance Monitoring and Cross-resistance to the Diamides in the Rice Stem Borer, Chilo suppressalis (Lepidoptera: Pyralidae)[J]. Chinese Journal OF Rice Science, 2017, 31(3): 307-314.

赵丹丹, 周丽琪, 张帅, 姚蓉, 邱运霞, 高聪芬. 二化螟对双酰胺类杀虫剂的抗药性监测和交互抗性研究[J]. 中国水稻科学, 2017, 31(3): 307-314.

Figures/Tables 5

References 30

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采集地点 Collection site				测定代次 Generation
湖南东安 Dong’an, Hunan Province	DA	04-02	蛹 Pupa	F₁
湖南攸县 Youxian, Hunan Province	YX	04-30	卵块 Egg	F₀
湖南芷江 Zhijiang, Hunan Province	ZJ	05-22	卵块 Egg	F₀
浙江苍南 Cangnan, Zhejiang Province	CN	04-11	老熟幼虫 Mature larvae	F₁
浙江瑞安 Ruian, Zhejiang Province	RA	04-25	卵块 Egg	F₀
浙江象山 Xiangshan, Zhejiang Province	XS	06-30	蛹 Pupa	F₁
浙江余姚 Yuyao, Zhejiang Province	YY	05-21	卵块 Egg	F₀
湖北孝感 Xiaogan, Hubei Province	XG	05-14	卵块 Egg	F₀
湖北荆州 Jingzhou, Hubei Province	JZ	04-04	老熟幼虫 Mature larvae	F₁
湖北监利 Jianli, Hubei Province	JL	08-26	卵块 Egg	F₀
安徽潜山 Qianshan, Anhui Province	QS	05-08	卵块 Egg	F₀
安徽庐江 Lujiang, Anhui Province	LJ	08-15	幼虫 Larvae	F₁
安徽徽州 Huizhou, Anhui Province	HZ	05-17	卵块 Egg	F₀
江西上高 Shanggao, Jiangxi Province	SG	04-05	老熟幼虫 Mature larvae	F₁
江西南城 Nancheng, Jiangxi Province	NC	04-11	卵块 Egg	F₀
江苏仪征 Yizheng, Jiangsu Province	YZ	05-27	卵块 Egg	F₀
江苏邗江 Hanjiang, Jiangsu Province	HJ	05-28	卵块 Egg	F₀
四川乐山 Leshan, Sichuan Province	LS	07-24	蛹 Pupa	F₁
四川绵阳 Mianyang, Sichuan Province	MY	08-30	幼虫 Larvae	F₁

采集地点 Collection site				测定代次 Generation
湖南东安 Dong’an, Hunan Province	DA	04-02	蛹 Pupa	F₁
湖南攸县 Youxian, Hunan Province	YX	04-30	卵块 Egg	F₀
湖南芷江 Zhijiang, Hunan Province	ZJ	05-22	卵块 Egg	F₀
浙江苍南 Cangnan, Zhejiang Province	CN	04-11	老熟幼虫 Mature larvae	F₁
浙江瑞安 Ruian, Zhejiang Province	RA	04-25	卵块 Egg	F₀
浙江象山 Xiangshan, Zhejiang Province	XS	06-30	蛹 Pupa	F₁
浙江余姚 Yuyao, Zhejiang Province	YY	05-21	卵块 Egg	F₀
湖北孝感 Xiaogan, Hubei Province	XG	05-14	卵块 Egg	F₀
湖北荆州 Jingzhou, Hubei Province	JZ	04-04	老熟幼虫 Mature larvae	F₁
湖北监利 Jianli, Hubei Province	JL	08-26	卵块 Egg	F₀
安徽潜山 Qianshan, Anhui Province	QS	05-08	卵块 Egg	F₀
安徽庐江 Lujiang, Anhui Province	LJ	08-15	幼虫 Larvae	F₁
安徽徽州 Huizhou, Anhui Province	HZ	05-17	卵块 Egg	F₀
江西上高 Shanggao, Jiangxi Province	SG	04-05	老熟幼虫 Mature larvae	F₁
江西南城 Nancheng, Jiangxi Province	NC	04-11	卵块 Egg	F₀
江苏仪征 Yizheng, Jiangsu Province	YZ	05-27	卵块 Egg	F₀
江苏邗江 Hanjiang, Jiangsu Province	HJ	05-28	卵块 Egg	F₀
四川乐山 Leshan, Sichuan Province	LS	07-24	蛹 Pupa	F₁
四川绵阳 Mianyang, Sichuan Province	MY	08-30	幼虫 Larvae	F₁

省份 Province	种群 Strain/Population	斜率(标准误) Slope (SE)	卡方值(自由度) χ2 (df)	LC₅₀(95%置信限) LC₅₀(95% confidence interval)/(mg·L^-1)	抗性倍数 Resistance ratio
	SS(敏感Susceptible)	2.473(0.290)	2.260(4)	0.693(0.549–0.858)	1.0
湖南Hunan	DA	2.061(0.247)	1.862(3)	3.456(2.581–4.546)	5.0
	YX	1.295(0.167)	0.785(3)	4.622(3.216–6.647)	6.7
	ZJ	1.336(0.161)	2.569(4)	3.455(2.317–5.047)	5.0
浙江Zhejiang	RA	1.298(0.168)	1.566(3)	3.306(2.269–4.729)	4.8
	XS	0.836(0.118)	0.999(4)	60.903(37.531–110.653)	87.9
	YY	1.485(0.220)	2.232(4)	97.772(69.794–139.611)	141.1
	CN	1.382(0.261)	0.213(3)	93.583(61.714–138.667)	135.0
湖北Hubei	XG	1.058(0.177)	0.035(3)	2.787(1.432–4.661)	4.0
	JZ	1.251(0.216)	1.211(3)	4.610(2.483–7.438)	6.7
	JL	0.871(0.126)	0.496(4)	10.969(6.403–18.745)	15.8
安徽Anhui	QS	1.156(0.159)	0.074(3)	5.230(3.533–7.867)	7.5
	LJ	0.907(0.151)	0.142(3)	9.917(6.139–18.483)	14.3
	HZ	0.963(0.186)	0.368(3)	2.100(1.026–3.940)	3.0
江西Jiangxi	SG	1.543(0.184)	1.239(3)	4.651(3.391–6.408)	6.7
江西Jiangxi	NC	1.237(0.141)	2.327(4)	15.220(10.700–22.330)	22.0
江苏Jiangsu	YZ	0.791(0.150)	0.910(3)	1.169(0.590–2.109)	1.7
江苏Jiangsu	HJ	0.941(0.162)	0.430(3)	1.169(0.619–1.997)	1.7
四川Sichuan	LS	2.061(0.247)	1.862(3)	3.456(2.581–4.546)	5.0
四川Sichuan	MY	0.985(0.153)	0.612(3)	0.806(0.473–1.255)	1.2

省份 Province	种群 Strain/Population	斜率(标准误) Slope (SE)	卡方值(自由度) χ2 (df)	LC₅₀(95%置信限) LC₅₀(95% confidence interval)/(mg·L^-1)	抗性倍数 Resistance ratio
	SS(敏感Susceptible)	2.473(0.290)	2.260(4)	0.693(0.549–0.858)	1.0
湖南Hunan	DA	2.061(0.247)	1.862(3)	3.456(2.581–4.546)	5.0
	YX	1.295(0.167)	0.785(3)	4.622(3.216–6.647)	6.7
	ZJ	1.336(0.161)	2.569(4)	3.455(2.317–5.047)	5.0
浙江Zhejiang	RA	1.298(0.168)	1.566(3)	3.306(2.269–4.729)	4.8
	XS	0.836(0.118)	0.999(4)	60.903(37.531–110.653)	87.9
	YY	1.485(0.220)	2.232(4)	97.772(69.794–139.611)	141.1
	CN	1.382(0.261)	0.213(3)	93.583(61.714–138.667)	135.0
湖北Hubei	XG	1.058(0.177)	0.035(3)	2.787(1.432–4.661)	4.0
	JZ	1.251(0.216)	1.211(3)	4.610(2.483–7.438)	6.7
	JL	0.871(0.126)	0.496(4)	10.969(6.403–18.745)	15.8
安徽Anhui	QS	1.156(0.159)	0.074(3)	5.230(3.533–7.867)	7.5
	LJ	0.907(0.151)	0.142(3)	9.917(6.139–18.483)	14.3
	HZ	0.963(0.186)	0.368(3)	2.100(1.026–3.940)	3.0
江西Jiangxi	SG	1.543(0.184)	1.239(3)	4.651(3.391–6.408)	6.7
江西Jiangxi	NC	1.237(0.141)	2.327(4)	15.220(10.700–22.330)	22.0
江苏Jiangsu	YZ	0.791(0.150)	0.910(3)	1.169(0.590–2.109)	1.7
江苏Jiangsu	HJ	0.941(0.162)	0.430(3)	1.169(0.619–1.997)	1.7
四川Sichuan	LS	2.061(0.247)	1.862(3)	3.456(2.581–4.546)	5.0
四川Sichuan	MY	0.985(0.153)	0.612(3)	0.806(0.473–1.255)	1.2

省份 Province	种群 Strain/Population	斜率(标准误) Slope (SE)	卡方值(自由度) χ2 (df)	LC₅₀(95%置信限) LC₅₀(95% confidence interval)/(mg·L^-1)	抗性倍数 Resistance ratio
	SS(敏感Susceptible)	2.172(0.283)	1.949(4)	0.217(0.173–0.266)	1.0
湖南Hunan	DA	1.037(0.161)	0.728(3)	0.245(0.140–0.387)	1.1
	YX	0.990(0.151)	0.178(3)	0.397(0.249–0.623)	1.8
	ZJ	0.879(0.146)	0.802(3)	0.158(0.095–0.266)	0.7
浙江Zhejiang	RA	1.232(0.165)	1.978(3)	0.234(0.153–0.338)	1.1
	XS	0.758(0.129)	1.859(4)	7.832(4.218–16.726)	36.1
	YY	1.611(0.262)	1.195(3)	3.253(2.221–4.421)	15.0
	CN	1.509(0.302)	0.154(3)	12.737(8.368–19.391)	58.7
湖北Hubei	XG	1.177(0.199)	0.706(3)	0.359(0.189–0.587)	1.7
	JZ	1.126(0.216)	2.201(3)	0.561(0.279–0.960)	2.6
	JL	0.880(0.133)	0.652(4)	1.742(1.014–3.109)	8.0
安徽Anhui	QS	1.082(0.159)	0.473(3)	0.231(0.142–0.348)	1.1
	LJ	0.943(0.149)	0.735(3)	0.621(0.391–1.036)	2.9
	HZ	0.985(0.199)	0.659(3)	0.267(0.136–0.530)	1.2
江西Jiangxi	SG	1.052(0.154)	0.599(3)	0.398(0.256–0.610)	1.8
江西Jiangxi	NC	0.889(0.119)	0.367(4)	1.535(0.979–2.566)	7.1
江苏Jiangsu	YZ	0.839(0.152)	0.531(3)	0.081(0.040–0.140)	0.4
江苏Jiangsu	HJ	0.953(0.167)	1.454(3)	0.113(0.058–0.193)	0.5
四川Sichuan	LS	1.037(0.161)	0.728(3)	0.245(0.140–0.387)	1.1